Abstract: . . .  start the aliquoting. The process should take about 30 minutes to complete. A1 D6 Rack 1 Wells A1- D6 A7 D12 Rack 2 Wells A7- D12 E1 H6 Rack 3 Wells E1- H6 E7 H12 Rack 4 Wells E7- H12 Page 13 Pharmacogenomics Protocol #030199 Drs. OConnor and Bhatnagar Original -6/5/03 Revision- 6/8/04 13. While the robot is working on the first plate get the second set of racks ready, filling them witht he second plates DNA tubes. When the first plate is done immediately seal the . . .
. . .  replace them with the next set of racks containing the DNA for the next DNA plate. Get the next robot started on the next set MAKING SURE YOU HAVE IMPORTED THE CORRECT H2O AND DNA VOLUMES FOR THE NEW PLATE. In the meantime you can close the first set of DNA tubes and put them away and start uncapping the third set of DNAs. 14. Repeat this process untill all of the DNA had been aliquoted from the stock tubes into DNA plates. 15. Please make sure that all of the DNA tubes are back in their original boxes in numerical order after you have completed aliquotting. . . .
. . .  step 4 to preformatted spreadsheets which will be used to tell the Biomek how much water and DNA to put into each well of your DNA plates. There are two preformatted spreadsheets used with the Biomek, 1 for the water volumes and 1 for the DNA volumes, and they can be found on the Hypertension Lab website. They must be saved in comma separated value format (.csv). They have the layout shown below: Page 9 Pharmacogenomics Protocol #030199 Drs. OConnor and Bhatnagar Original -6/5/03 Revision- 6/8/04 Water: Pre-formatted . . .
. . .  Pharmacogenomics Protocol #030199 Drs. OConnor and Bhatnagar Original -6/5/03 Revision- 6/8/04 Pharmacogenomics and Hypertension Draft Protocols June 17, 2004 (1) Mail Recruitment HTN Clinical lab (2) Consent and Sample Returned HTN Clinical lab (3) DNA Extraction and Quantification Stein HTN lab (4) DNA Prep for Genotyping Genomics Core/ Stein HTN lab ( . . .
. . .  -6/5/03 Revision- 6/8/04 Pharmacogenomics #030199 Drs. OConnor and Bhatnagar -Bar-coded mouthwash samples are refrigerated, and stable for many days. -These samples are processed through extraction protocol at http://medicine.ucsd.edu/ hypertension . Click on Protocols. <Puregene DNA Purification Kit (Gentra)>. Cell Lysis and Extraction: -Bar-coded samples are lysed every week, sometimes twice a week depending on the number of samples received. -Lysed samples are stable after adding cell lysis solution, and transferred . . .
--3000,5,300,3240,16526